Get to know how Amperia™ fits into your protein quantification workflow.
1. What types of proteins can I measure with Amperia™?
Amperia™ is designed for total protein quantification and is compatible with a wide range of analytes, including monoclonal antibodies (mAbs), AAV capsid proteins, and His-tagged recombinant proteins. Dedicated kits are available for each application to ensure optimal accuracy and reproducibility.
The platform is continually evolving, with additional assay types and formats in development — expanding compatibility with other protein classes and workflows.
2. How does the Amperia™ system work?
Amperia™ uses Redox Electrochemical Detection (RED) to quantify proteins based on binding interactions at the sensor surface. Each disposable sensor strip includes four individual probes, enabling multiple samples or replicates to be measured in a single run.
Proteins in solution bind to functionalised surfaces on the sensor, where they participate in a surface-coupled enzymatic reaction that produces an electrochemical signal. This signal is detected and quantified without the need for secondary antibodies, optical components, or complex wash steps.
3. What is the quantification range of the system, and how is it reported?
The Amperia™ system is designed to support total protein quantification across a range of concentrations, depending on the assay. For example, monoclonal antibody and His-tagged protein assays typically report values in µg/mL, while AAV assays report capsid concentration, such as viral particles per mL (vp/mL).
While standards are not included in the assay kits, each protocol provides guidance on selecting and preparing appropriate standards, along with a recommended dilution range. This enables users to generate a standard curve suitable for their specific sample type and application.
4. How many tests can I run in a single run?
The Amperia™ system includes two plate positions (left and right), each holding up to five sensor strips — for a total of ten strips per run. Each strip has four measurement probes, and the sensor head automatically picks up one strip at a time.
The total number of tests you can perform in a run — including samples, standards, and controls — depends on the assay type:
- Up to 64 tests for monoclonal antibody (mAb) assays (probes can be regenerated within the run)
- Up to 40 tests for AAV total capsid quantification
- Up to 32 tests for His-tagged protein assays
The Amperia™ software guides probe allocation and tracks usage automatically, ensuring reliable throughput and data quality.
5. Can I use crude or in-process samples?
Yes. Amperia™ assays are compatible with many crude, in-process, or unpurified samples, including cell culture supernatants and lysates.
However, matrix effects can impact signal quality — especially in AAV and His-tag assays. For best results, we recommend:
- Gentle clarification (e.g. centrifugation or filtration)
- Consistent buffer conditions
- Including controls matched to the sample matrix
The system is designed to be robust, but thoughtful sample prep helps maintain accuracy.
6. Is Amperia™ suitable for regulated or clinical workflows?
Amperia™ is intended for research use only (RUO) and is not validated for clinical diagnostics or GMP-compliant release testing.
However, the system is well-suited to early-stage development, process characterisation, and method exploration — where fast, reliable protein quantification supports decision-making before formal assay validation.